Review



alp staining kit  (Beyotime)


Bioz Verified Symbol Beyotime is a verified supplier
Bioz Manufacturer Symbol Beyotime manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Beyotime alp staining kit
    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of <t>ALP-positive</t> area. (C) Representative image of <t>ALP</t> <t>staining.</t> (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Alp Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 3882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip+nbt+alp+staining+kit/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pmc13683671-266-16-19
    Average 99 stars, based on 3882 article reviews
    alp staining kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Natural polysaccharide-engineered POP@ZIF-67 nanoparticles coordinate antibacterial activity, macrophage polarization, and osteogenesis for chronic osteomyelitis therapy"

    Article Title: Natural polysaccharide-engineered POP@ZIF-67 nanoparticles coordinate antibacterial activity, macrophage polarization, and osteogenesis for chronic osteomyelitis therapy

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2026.1935416

    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of ALP-positive area. (C) Representative image of ALP staining. (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Figure Legend Snippet: Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of ALP-positive area. (C) Representative image of ALP staining. (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Techniques Used: Infection, CCK-8 Assay, Cell Viability Assay, Analysis, Staining, Expressing

    Related Articles

    other:

    Article Title: Immunomodulatory Hydrogel Coating with SeNPs and Lithium Silicate Synergistically Promotes Osseointegration and Prevents Infection on Titanium Implants.
    Article Snippet: Quantitative evaluation of angiogenic structures, including total tube length and number of branch points were quantified using ImageJ software.

    Staining:

    Article Title: Mechanism and regulatory strategy study on promoting vascularized bone regeneration via intracellular zinc ion transport
    Article Snippet: Subsequently, the images of the cells were captured using a microscope (OLYMPUS) to visualize and analyze the uptake of CD90@ZIF-8-ICG. .. A BCIP/NBT ALP staining kit (Beyotime) was used for staining. ..

    Article Title: Leveraging solid-liquid interaction to fabricate drug-microsphere in site encapsulated bone-repair scaffolds.
    Article Snippet: The total DNA content was quantified with a Picogreent dye-based fluorescent DNA Quantification Kit (Invitrogen, USA) for normalization of ALP activity. .. The ALP staining was conducted using a BCIP/NBT ALP staining kit (Beyotime, China). ..

    Article Title: Evaluating the Effects of Selective Serotonin Reuptake Inhibitors on Osteogenesis of Periodontal Stem Cells: An In Vitro Study
    Article Snippet: .. Alkaline phosphatase (ALP) staining was performed on day 7 using a BCIP/NBT ALP staining kit (Beyotime), and Alizarin Red S (ARS) staining was performed on day 21 using an ARS staining kit (Solarbio) to assess osteogenic differentiation. ..

    Article Title: Bimetallic MOF-engineered 3D-printed PEEK implants orchestrate osteoregeneration via macrophage reprogramming
    Article Snippet: 3D-printed polyetheretherketone (PEEK) scaffolds hold great promise for personalized bone repair owing to their excellent mechanical properties and highly tunable porous architectures.. However, their intrinsic bioinertness severely limits osseointegration and long-term clinical efficacy.. We have developed an innovative bioactive scaffold (SIM@MPSP) by engineering a multifunctional biointerface onto 3D-printed porous PEEK.

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: Luria-Bertani (LB) broth, crystal violet dye, penicillin-streptomycin solution (100 U/mL penicillin, 100 μg/mL streptomycin), Mouse IL-6 ELISA Kit, Mouse IL-10 ELISA Kit, and histological staining kits (H&E and Masson) were purchased from Solarbio (China). .. DNase I (1 U/μL), bacterial viability staining kit, DiI plasma membrane stain, Calcein-AM/PI double staining kit, and BCIP/NBT ALP staining kit were purchased from Beyotime Biotechnology (China). .. Hoechst 33342 nuclear stain, TRIzol reagent, and secondary antibodies for immunostaining were purchased from Thermo Fisher Scientific (USA).

    Article Title: Synergistic dual chemophysical FeCu-MOF scaffold with PEMF stimulation drives angiogenic-osteogenic coupling for bone regeneration
    Article Snippet: .. ALP staining was then performed using a BCIP/NBT ALP Staining Kit (Beyotime, Shanghai, China) following the manufacturer's protocol. ..

    ALP Assay:

    Article Title: Evaluating the Effects of Selective Serotonin Reuptake Inhibitors on Osteogenesis of Periodontal Stem Cells: An In Vitro Study
    Article Snippet: .. Alkaline phosphatase (ALP) staining was performed on day 7 using a BCIP/NBT ALP staining kit (Beyotime), and Alizarin Red S (ARS) staining was performed on day 21 using an ARS staining kit (Solarbio) to assess osteogenic differentiation. ..

    Article Title: Bimetallic MOF-engineered 3D-printed PEEK implants orchestrate osteoregeneration via macrophage reprogramming
    Article Snippet: 3D-printed polyetheretherketone (PEEK) scaffolds hold great promise for personalized bone repair owing to their excellent mechanical properties and highly tunable porous architectures.. However, their intrinsic bioinertness severely limits osseointegration and long-term clinical efficacy.. We have developed an innovative bioactive scaffold (SIM@MPSP) by engineering a multifunctional biointerface onto 3D-printed porous PEEK.

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: Luria-Bertani (LB) broth, crystal violet dye, penicillin-streptomycin solution (100 U/mL penicillin, 100 μg/mL streptomycin), Mouse IL-6 ELISA Kit, Mouse IL-10 ELISA Kit, and histological staining kits (H&E and Masson) were purchased from Solarbio (China). .. DNase I (1 U/μL), bacterial viability staining kit, DiI plasma membrane stain, Calcein-AM/PI double staining kit, and BCIP/NBT ALP staining kit were purchased from Beyotime Biotechnology (China). .. Hoechst 33342 nuclear stain, TRIzol reagent, and secondary antibodies for immunostaining were purchased from Thermo Fisher Scientific (USA).

    Article Title: Synergistic dual chemophysical FeCu-MOF scaffold with PEMF stimulation drives angiogenic-osteogenic coupling for bone regeneration
    Article Snippet: .. ALP staining was then performed using a BCIP/NBT ALP Staining Kit (Beyotime, Shanghai, China) following the manufacturer's protocol. ..

    Differentiation Assay:

    Article Title: Evaluating the Effects of Selective Serotonin Reuptake Inhibitors on Osteogenesis of Periodontal Stem Cells: An In Vitro Study
    Article Snippet: .. Alkaline phosphatase (ALP) staining was performed on day 7 using a BCIP/NBT ALP staining kit (Beyotime), and Alizarin Red S (ARS) staining was performed on day 21 using an ARS staining kit (Solarbio) to assess osteogenic differentiation. ..

    Clinical Proteomics:

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: Luria-Bertani (LB) broth, crystal violet dye, penicillin-streptomycin solution (100 U/mL penicillin, 100 μg/mL streptomycin), Mouse IL-6 ELISA Kit, Mouse IL-10 ELISA Kit, and histological staining kits (H&E and Masson) were purchased from Solarbio (China). .. DNase I (1 U/μL), bacterial viability staining kit, DiI plasma membrane stain, Calcein-AM/PI double staining kit, and BCIP/NBT ALP staining kit were purchased from Beyotime Biotechnology (China). .. Hoechst 33342 nuclear stain, TRIzol reagent, and secondary antibodies for immunostaining were purchased from Thermo Fisher Scientific (USA).

    Membrane:

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: Luria-Bertani (LB) broth, crystal violet dye, penicillin-streptomycin solution (100 U/mL penicillin, 100 μg/mL streptomycin), Mouse IL-6 ELISA Kit, Mouse IL-10 ELISA Kit, and histological staining kits (H&E and Masson) were purchased from Solarbio (China). .. DNase I (1 U/μL), bacterial viability staining kit, DiI plasma membrane stain, Calcein-AM/PI double staining kit, and BCIP/NBT ALP staining kit were purchased from Beyotime Biotechnology (China). .. Hoechst 33342 nuclear stain, TRIzol reagent, and secondary antibodies for immunostaining were purchased from Thermo Fisher Scientific (USA).

    Double Staining:

    Article Title: Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS 2 in the treatment of pyogenic osteomyelitis
    Article Snippet: Luria-Bertani (LB) broth, crystal violet dye, penicillin-streptomycin solution (100 U/mL penicillin, 100 μg/mL streptomycin), Mouse IL-6 ELISA Kit, Mouse IL-10 ELISA Kit, and histological staining kits (H&E and Masson) were purchased from Solarbio (China). .. DNase I (1 U/μL), bacterial viability staining kit, DiI plasma membrane stain, Calcein-AM/PI double staining kit, and BCIP/NBT ALP staining kit were purchased from Beyotime Biotechnology (China). .. Hoechst 33342 nuclear stain, TRIzol reagent, and secondary antibodies for immunostaining were purchased from Thermo Fisher Scientific (USA).



    Similar Products

    99
    Beyotime alp staining kit
    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of <t>ALP-positive</t> area. (C) Representative image of <t>ALP</t> <t>staining.</t> (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Alp Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip+nbt+alp+staining+kit/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pmc13683671-266-16-19
    Average 99 stars, based on 1 article reviews
    alp staining kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime alkaline phosphatase alp staining kit
    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of <t>ALP-positive</t> area. (C) Representative image of <t>ALP</t> <t>staining.</t> (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Alkaline Phosphatase Alp Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip+nbt+alp+staining+kit/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/10__1093_slash_rb_slash_rbag200-57-4-29
    Average 99 stars, based on 1 article reviews
    alkaline phosphatase alp staining kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime alkaline phosphatase alp staining
    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of <t>ALP-positive</t> area. (C) Representative image of <t>ALP</t> <t>staining.</t> (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Alkaline Phosphatase Alp Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip+nbt+alp+staining+kit/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/10__1016_slash_j__cej__2026__182013-302-19-29
    Average 99 stars, based on 1 article reviews
    alkaline phosphatase alp staining - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime bcip nbt alp staining kit
    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of <t>ALP-positive</t> area. (C) Representative image of <t>ALP</t> <t>staining.</t> (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Bcip Nbt Alp Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip+nbt+alp+staining+kit/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pmc12720349-304-17-24
    Average 99 stars, based on 1 article reviews
    bcip nbt alp staining kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime bcip nbt alkaline phosphatase alp staining kit
    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of <t>ALP-positive</t> area. (C) Representative image of <t>ALP</t> <t>staining.</t> (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Bcip Nbt Alkaline Phosphatase Alp Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip+nbt+alp+staining+kit/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pm41687523-93-14-20
    Average 99 stars, based on 1 article reviews
    bcip nbt alkaline phosphatase alp staining kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    Beyotime bcip nbt alp stain development kit
    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of <t>ALP-positive</t> area. (C) Representative image of <t>ALP</t> <t>staining.</t> (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.
    Bcip Nbt Alp Stain Development Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bcip+nbt+alp+staining+kit/BCIP-NBT+Alkaline+Phosphatase+Color+Development+Kit/pm41528647-89-6-11
    Average 99 stars, based on 1 article reviews
    bcip nbt alp stain development kit - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of ALP-positive area. (C) Representative image of ALP staining. (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Natural polysaccharide-engineered POP@ZIF-67 nanoparticles coordinate antibacterial activity, macrophage polarization, and osteogenesis for chronic osteomyelitis therapy

    doi: 10.3389/fbioe.2026.1935416

    Figure Lengend Snippet: Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of ALP-positive area. (C) Representative image of ALP staining. (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: For alkaline phosphatase (ALP) staining, cells were fixed after early osteogenic induction and stained with an ALP staining kit (Beyotime, Shanghai, China).

    Techniques: Infection, CCK-8 Assay, Cell Viability Assay, Analysis, Staining, Expressing